Superoxide Dismutase Reduces Creatinine and NGAL by Restoring Oxidative Balance during Sepsis

Jufitriani Ismy; Maimun Syukri; Dessy R. Emril; Nanan Sekarwana; Jufriady Ismy

doi:10.28991/ESJ-2022-06-02-06

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Jufitriani Ismy 1) Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. 2) Department of Pediatrics, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23111,, Indonesia
Maimun Syukri
maimun_62@unsyiah.ac.id
Department of Internal Medicine, Universitas Syiah Kuala, Banda Aceh, 23111,, Indonesia
Dessy R. Emril Department of Neurology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, 23111,, Indonesia
Nanan Sekarwana Department of Pediatrics, Faculty of Medicine, Universitas Islam Bandung, Bandung, 40161,, Indonesia
Jufriady Ismy Departement of Surgery, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh 23111,, Indonesia

Vol. 6 No. 2 (2022): April

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Sepsis-associated overproduction of reactive oxygen species (ROS) and nitric oxide (NO) during pathogen infection leads to overwhelming oxidative stress, which has been recognized as a primary contributor to acute kidney injury (AKI). Hence, antioxidant therapy has been widely explored in order to find an effective treatment for sepsis-related AKI, in particular by using endogenous antioxidant – superoxide dismutase (SOD). We assessed the effect of oral SOD on the alteration of AKI biomarkers (creatinine and Neutrophil Gelatinase-Associated Lipocalin – NGAL) in endotoxin-induced septic murine. The animals were assigned as a healthy control, a septic control, and three treatment groups (250, 500, and 1000 IU oral SOD). Treatment of SOD was carried out by force-feeding for 16 weeks prior to intraperitoneal injection of lipopolysaccharide (LPS). The sepsis was assessed using the murine sepsis score (MSS) after 12 hours post-LPS injection, where the changes in plasma SOD, ROS, NO, creatinine, and NGAL were measured by enzyme-linked immunosorbent assay (ELISA). During sepsis, SOD was significantly decreased from its baseline level while other biomarkers were significantly increased (p<0.05) – except for NGAL. MSS exhibited a declining trend in SOD dosage-dependent manner, and was significantly different with that of septic control group at SOD dosage of 1000 IU (p<0.05). SOD treatment with a dosage as low as 250 IU could prevent the abnormal expression of the tested biomarkers during sepsis. There were significant reduction of plasma ROS, NO, creatine and NGAL in rats treated with 1000 IU SOD. Our study suggests the protective effect of SOD against sepsis-induced AKI by scavenging ROS and NO.

Doi: 10.28991/ESJ-2022-06-02-06

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Ismy, J., Syukri, M., Emril, D. R., Sekarwana, N., & Ismy, J. (2022). Superoxide Dismutase Reduces Creatinine and NGAL by Restoring Oxidative Balance during Sepsis. Emerging Science Journal, 6(2), 286–294. https://doi.org/10.28991/ESJ-2022-06-02-06

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Superoxide Dismutase Reduces Creatinine and NGAL by Restoring Oxidative Balance during Sepsis

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Superoxide Dismutase Reduces Creatinine and NGAL by Restoring Oxidative Balance during Sepsis

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Optical and Structural Characterization of Bi2FexNbO7 Nanoparticles for Environmental Applications

Impediments of Green Finance Adoption System: Linking Economy and Environment

Digital Transformation: Opportunities and Challenges for Leaders in the Emerging Countries in Response to Covid-19 Pandemic

Thermal Regeneration and Reuse of Carbon and Glass Fibers from Waste Composites